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cyto flex flow cytometer  (Danaher Inc)


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    Danaher Inc cyto flex flow cytometer
    Cyto Flex Flow Cytometer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 9798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyto+flex+flow+cytometer/CytoFLEX+Flow+Cytometers/pm41761283-89-9-13
    Average 99 stars, based on 9798 article reviews
    cyto flex flow cytometer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Irradiation:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Electron Microscopy:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Transmission Assay:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Microscopy:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Zeta Potential Analyzer:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Spectrophotometry:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Concentration Assay:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Flow Cytometry:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Identification of blood immunological biomarkers of SARS-CoV-2 infection during pandemic in Poland
    Article Snippet: .. The prepared samples were immediately analyzed using a Cyto FLEX Flow Cytometer (Beckman Coulter, USA) and processed with FCAP Array Software Version 3.0.1 (BD Biosciences, USA). .. GraphPad Prism software (v. 10.4.1, La Jolla, CA, USA) was used for statistical analysis.

    Article Title: Pre-cooling of boar semen before transport in a hot environment enhances biosafety
    Article Snippet: .. Flow cytometry was conducted following the protocols outlined in reference (Paschoal et al., ), using the Cyto Flex flow cytometer (Beckman Coulter GmbH, Krefeld, Germany) equipped with three lasers (488, 638, 405 nm). .. Gating was executed with CytExpert 2.4 Software (Beckman Coulter GmbH).

    Article Title: Bacteroides coprocola protects dopaminergic neurons in rotenone-induced Parkinson’s disease mouse model by modulating gut microbiota dysbiosis and inhibiting the NLRP3 signaling pathway
    Article Snippet: .. The prepared samples were measured and analyzed using a Cyto FLEX flow cytometer (Beckman Coulter, Brea, CA). ..

    Article Title: Integrating network pharmacology and metabolomics to explore the mechanism of Xihuang Pill in exerting anti-tumor immune effects against pancreatic ductal adenocarcinoma.
    Article Snippet: Ethnopharmacological relevance: Xihuang Pill (XHP), a traditional Chinese medicine formula, has demonstrated anti-pancreatic ductal adenocarcinoma (PDAC) efficacy in prior studies.. However, its effects on tumor-associated macrophages (TAMs) and underlying mechanisms remain unclear.. Aim of the study: This study aimed to elucidate the mechanism of XHP in exerting anti-tumor immune effects against PDAC through network pharmacology, widely targeted metabolomics, and in vitro and in vivo experiments.

    Article Title: Bacteroides coprocola protects dopaminergic neurons in rotenone-induced Parkinson's disease mouse model by modulating gut microbiota dysbiosis and inhibiting the NLRP3 signaling pathway.
    Article Snippet: .. The prepared samples were measured and analyzed using a Cyto FLEX flow cytometer (Beckman Coulter, Brea, CA). ..

    Article Title: Nanoformulations Downregulating METTL16 Combined with mRNA Tumor Vaccines Suppress Triple-Negative Breast Cancer and Prevent Metastasis
    Article Snippet: .. Post incubation, analysis using a Cyto-FLEX flow cytometer (Beckman Coulter). ..

    Fluorescence:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Staining:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Imaging:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Functional Assay:

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer.
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probewasproceededbyUV2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    Article Title: Peptide-functionalized membrane camouflage for endogenous H 2 S-induced photothermal immunotherapy of orthotopic colorectal cancer
    Article Snippet: The fluorescence imaging system was constructed by our laboratory; MDL-III-808-2.5 W laser (Changchun New Industries Optoelectronics Tech. .. Co., Ltd, China) was used for laser irradiation; electron microscopy characterization was performed on Hitachi 120 kV HT7700 transmission electron microscope (Hitachi, Japan); particle size and zeta potential of the probe were characterized by Nano-ZS90 nanometre (Malvern, UK); vacuum concentrator (Ependorf, Germany) was to purify the probe; absorption spectra of probe was proceeded by UV-2550 ultraviolet-visible spectrophotometer (Shimadzu, Japan); x’pert3 power X-ray diffractometer (Panalytical BV, Netherlands) was used for the structure analysis of nanoparticle; WFX-200 atomic absorption spectrophotometer (Beijing Beifen-Ruili Analytical Intrument Co., Ltd., China) was to detect concentration of Co; quantitative analysis of probe-induced apoptosis were obtained through Cyto FLEX flow cytometer (Beckman Coulter, USA); FV3000 confocal microscope (Olympus, Japan) was to acquire cell fluorescence staining imaging; changes of characteristic functional groups during synthesis were verified by Vertex 70 infra-red spectrometer (Bruker, Germany); absorbance detection of well plate was carried out by flexStation3 microplate reader (Molecular Devices, USA). ..

    FCAP Assay:

    Article Title: Identification of blood immunological biomarkers of SARS-CoV-2 infection during pandemic in Poland
    Article Snippet: .. The prepared samples were immediately analyzed using a Cyto FLEX Flow Cytometer (Beckman Coulter, USA) and processed with FCAP Array Software Version 3.0.1 (BD Biosciences, USA). .. GraphPad Prism software (v. 10.4.1, La Jolla, CA, USA) was used for statistical analysis.

    Software:

    Article Title: Identification of blood immunological biomarkers of SARS-CoV-2 infection during pandemic in Poland
    Article Snippet: .. The prepared samples were immediately analyzed using a Cyto FLEX Flow Cytometer (Beckman Coulter, USA) and processed with FCAP Array Software Version 3.0.1 (BD Biosciences, USA). .. GraphPad Prism software (v. 10.4.1, La Jolla, CA, USA) was used for statistical analysis.

    Article Title: Integrating network pharmacology and metabolomics to explore the mechanism of Xihuang Pill in exerting anti-tumor immune effects against pancreatic ductal adenocarcinoma.
    Article Snippet: Ethnopharmacological relevance: Xihuang Pill (XHP), a traditional Chinese medicine formula, has demonstrated anti-pancreatic ductal adenocarcinoma (PDAC) efficacy in prior studies.. However, its effects on tumor-associated macrophages (TAMs) and underlying mechanisms remain unclear.. Aim of the study: This study aimed to elucidate the mechanism of XHP in exerting anti-tumor immune effects against PDAC through network pharmacology, widely targeted metabolomics, and in vitro and in vivo experiments.

    Incubation:

    Article Title: Nanoformulations Downregulating METTL16 Combined with mRNA Tumor Vaccines Suppress Triple-Negative Breast Cancer and Prevent Metastasis
    Article Snippet: .. Post incubation, analysis using a Cyto-FLEX flow cytometer (Beckman Coulter). ..



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